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Updated: Aug 30, 2026

A Cell Free Assay to Study Chromatin Decondensation at the End of Mitosis
Published on: December 19, 2015
[Induction of nuclear envelope formation around individual chromosomes under impact of hypotonic shock]
S Iu Kurchashova1, V V Filimonenko, P V Gulak
1Department of Electron Microscopy, Research Institute of Physico-Chemical Biology after A. N. Belozersky, Moscow State University.
Abstract:
In the present work we have studied the distribution of some proteins participating in the nuclear envelope assembly (lamins A/C, B and LAP2 alpha) in mitotic cells and after hypotonic treatment with 15% Hank's solution. In untreated cells, these proteins are localized in the nuclei of interphase cells migrate to the cytoplasm during mitosis. Hypotonic treatment of interphase, prophase and telophase cells does not lead to considerable relocalization of lamins A/C and B. However, unlike normal mitosis, in prometaphase and metaphase cells their chromosomes acquire affinity to lamins and LAP2 alpha. Comparative analysis of lamins and LAP2 alpha distribution have revealed that chromosomes have special sites for binding with different proteins.
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