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Expression of Japanese quail ovalbumin in Saccharomyces cerevisiae
L Krizková1, J Jeloková, J Klaudíny
1Laboratory of Genetic Engineering, Slovak Academy of Sciences, Bratislava.
Folia Microbiologica
|January 1, 1992
Abstract:
A cDNA sequence coding for Japanese quail ovalbumin was used for the construction of expression plasmid under the ADH1 promoter of the yeast shuttle vector pVT101-U. The resulting recombinant expression vector pJK2 was used for the transformation of Saccharomyces cerevisiae. Expression of quail ovalbumin in yeast cells was demonstrated by Western blotting followed by immunochemical detection.