Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Dynamic compression of cartilage constructs engineered from expanded human articular chondrocytes.

O Démarteau1, D Wendt, A Braccini

  • 1Department of Surgery, University Hospital, Basel, Switzerland.

Biochemical and Biophysical Research Communications
|October 3, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Azimuthal Correlations within Exclusive Dijets with Large Momentum Transfer in Photon-Lead Collisions.

Physical review letters·2023
Same author

Measurements of the associated production of a W boson and a charm quark in proton-proton collisions at <math> </math>.

The European physical journal. C, Particles and fields·2022
Same author

First Search for Exclusive Diphoton Production at High Mass with Tagged Protons in Proton-Proton Collisions at sqrt[s]=13  TeV.

Physical review letters·2022
Same author

Search for low-mass dilepton resonances in Higgs boson decays to four-lepton final states in proton-proton collisions at <math> </math>.

The European physical journal. C, Particles and fields·2022
Same author

Using Z Boson Events to Study Parton-Medium Interactions in Pb-Pb Collisions.

Physical review letters·2022
Same author

Search for strongly interacting massive particles generating trackless jets in proton-proton collisions at <math> </math>.

The European physical journal. C, Particles and fields·2022
Same journal

Quercetin suppresses TGF-β1-induced proliferation and migration of vascular smooth muscle cells via the Smad2/3/MMP-9 signaling axis.

Biochemical and biophysical research communications·2026
Same journal

Biosynthesis, characterization and biological potential of microbe-mediated silver nanoparticles using thermophilic actinomycetes, Streptomyces nigra.

Biochemical and biophysical research communications·2026
Same journal

COP9 signalosome 8 mediated autophagy drives proliferation, invasion, and metastasis in pancreatic ductal adenocarcinoma.

Biochemical and biophysical research communications·2026
Same journal

Tumor budding in colorectal cancer: partial EMT, microenvironmental remodeling, and metastatic competence.

Biochemical and biophysical research communications·2026
Same journal

Exploring the therapeutic versatility and multitarget pharmacological potential of acyl hydrazone-hydrazide scaffolds.

Biochemical and biophysical research communications·2026
Same journal

The plasma membrane H<sup>+</sup>-ATPase OSA2 negatively regulates salt tolerance in rice seedlings.

Biochemical and biophysical research communications·2026
See all related articles

Mechanical loading impacts chondrocyte metabolism in engineered cartilage. Dynamic compression stimulates glycosaminoglycan (GAG) formation in mature tissues, dependent on existing GAG content, not cell differentiation stage.

Area of Science:

  • Biomedical Engineering
  • Tissue Engineering
  • Cartilage Biology

Background:

  • Mechanical loading influences chondrocyte metabolic activity in 3D scaffolds.
  • Engineered cartilaginous constructs offer a model to study chondrocyte responses.

Purpose of the Study:

  • To investigate if the developmental stage of engineered cartilaginous constructs affects the response to dynamic compression on glycosaminoglycan (GAG) metabolism.
  • To determine the relationship between construct maturation and GAG metabolism under mechanical stimulation.

Main Methods:

  • Cultured adult human articular chondrocytes in Polyactive foams for 3-14 days to achieve varying maturation stages.
  • Applied dynamic compression for 3 days to engineered cartilaginous constructs.
  • Quantified changes in GAG synthesis, accumulation, and release, and measured collagen type II mRNA expression.

Related Experiment Videos

Main Results:

  • Changes in GAG metabolism were positively correlated with the pre-loading GAG content of the constructs.
  • Dynamic compression stimulated GAG formation only in the most developed (mature) cartilaginous tissues.
  • No correlation was found between GAG metabolism changes and collagen type II mRNA expression.

Conclusions:

  • The response of chondrocytes to dynamic compression in engineered tissues is primarily regulated by the extracellular matrix content, not the stage of cell differentiation.
  • Tissue maturation is a critical factor in determining the effectiveness of mechanical loading for stimulating GAG metabolism in engineered cartilage.