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Effect of Pasteurella multocida toxin on bone resorption in vitro
R Felix1, H Fleisch, P L Frandsen
1Department of Pathophysiology, University of Berne, Switzerland.
Infection and Immunity
|December 1, 1992
Summary
Pasteurella multocida toxin (PMT) stimulates bone resorption in vitro, a key factor in pig atrophic rhinitis. This effect was dose-dependent and could be inhibited by calcitonin, suggesting PMT
Area of Science:
- Veterinary Pathology
- Molecular Biology
- Bone Physiology
Background:
- Progressive atrophic rhinitis in pigs is primarily caused by Pasteurella multocida toxin (PMT).
- PMT's role in bone pathology, particularly bone resorption, requires further investigation.
Purpose of the Study:
- To investigate the in vitro effect of PMT on bone resorption.
- To elucidate the mechanisms underlying PMT-induced bone resorption.
Main Methods:
- In vitro bone resorption assays using murine calvaria and long bone cultures.
- Measurement of calcium and beta-glucuronidase release as indicators of bone resorption.
- Assessment of the effects of calcitonin and prostaglandin synthesis inhibitors.
Main Results:
- PMT significantly stimulated bone resorption in both murine calvaria and long bone cultures.
- The maximal effect was observed at a PMT concentration of 5 ng/ml, showing a clear dose-response relationship.
- Calcitonin completely inhibited PMT-induced bone resorption, while prostaglandin synthesis inhibitors partially suppressed it.
Conclusions:
- PMT directly stimulates bone resorption in vitro.
- The findings suggest a potential mechanism for PMT's role in the pathogenesis of atrophic rhinitis.
- Further research is needed to determine if PMT acts directly on bone cells or indirectly via nasal cavity cells.