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Related Experiment Videos

[Reliability and general practice value of 2 rapid Streptococcus A tests].

N Schmuziger1, S Schneider, R Frei

  • 1HNO-Universitätsklinik, Kantonspital Basel, Germany. nschmuziger@uhbs.ch

HNO
|October 3, 2003
PubMed
Summary
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Rapid antigen detection assays for Group A Streptococcus (GAS) show high specificity but moderate sensitivity. Both Strep A OIA MAX and Strep A Plus offer similar reliability for diagnosing pharyngitis, though Strep A Plus is simpler to use.

Area of Science:

  • Clinical microbiology
  • Infectious disease diagnostics

Background:

  • Rapid antigen detection assays for Group A Streptococcus (GAS) are widely used for diagnosing pharyngitis.
  • Existing assays exhibit variable sensitivity and specificity compared to throat cultures.
  • Contradictory results have been reported for newer optical immunoassay (OIA) systems.

Purpose of the Study:

  • To compare the performance of the Strep A OIA MAX with the established Strep A Plus immunoassay in patients with acute pharyngitis.
  • To evaluate the diagnostic accuracy, procedural steps, and turnaround time of both rapid assays.

Main Methods:

  • A comparative study involving 65 patients diagnosed with acute pharyngitis.
  • Standard throat culture was used as the reference standard.
  • Enhanced broth culturing and nucleic acid hybridization assays (Gen-Probe) were employed for discrepant results.

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Main Results:

  • Both Strep A OIA MAX and Strep A Plus demonstrated comparable sensitivity (78.3%).
  • Specificities were high for both assays: 95.2% for Strep A OIA MAX and 100% for Strep A Plus.
  • Strep A Plus required fewer procedural steps (4) and offered faster results (4-5 min) compared to Strep A OIA MAX (9 steps, 9-10 min).

Conclusions:

  • Both rapid immunoassays provide similar diagnostic reliability for GAS detection.
  • Strep A Plus offers a simpler and faster testing procedure than Strep A OIA MAX.
  • Neither assay is sufficiently sensitive to replace the need for confirmatory throat cultures.