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Visualizing Antigen Specific CD4+ T Cells using MHC Class II Tetramers
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Flow cytometric enumeration of Class I HLA-restricted, peptide-specific CD8+ T lymphocytes using tetramer technology

J Kraan1, M Arroz, M Keeney

  • 1Department of Internal Oncology, Erasmus MC, Daniel Den Hoed Cancer Centre, Rotterdam, The Netherlands.

Journal of Biological Regulators and Homeostatic Agents
|October 4, 2003
PubMed
Abstract

No abstract available in PubMed .

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Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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