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Updated: Aug 30, 2026

Measuring the Shape and Size of Activated Sludge Particles Immobilized in Agar with an Open Source Software Pipeline
Published on: January 30, 2019
Image analysis as a monitoring tool for activated sludge properties in lab-scale installations
R Jenné1, E N Banadda, N Philips
1Department of Chemical Engineering, BioTeC-Bioprocess Technology and Control, Katholieke Universiteit Leuven, Kasteelpart Arenberg 22, Heverlee, Belgium.
Abstract:
An important step in the battle against filamentous bulking is the development of a monitoring system for activated sludge properties. Therefore, a fully automatic image analysis method for recognizing and characterizing flocs and filaments in activated sludge images has been developed. This procedure has been subsequently used to monitor activated sludge properties in a lab-scale installation. The results of a 100-days experiment indicate that the image information correlates well with the evolution of standard settling properties, in this case the Sludge Volume Index. It is shown that, at the onset of severe filamentous bulking, there is an increase in total filament length on the one hand, and a significant change in floc shape on the other hand.

