Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

High-throughput screening of structural proteomics targets using NMR.

Leonor M P Galvão-Botton1, Angela M Katsuyama, Cristiane R Guzzo

  • 1Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, CEP 05508-900, SP, São Paulo, Brazil.

FEBS Letters
|October 7, 2003
PubMed
Summary

This study introduces a rapid method using NMR to screen proteins from Xanthomonas axonopodis pv citri. It helps prioritize which proteins are well-folded for future structural studies.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

p53-associated metabolic checkpoint regulation in a tick embryo-derived cell line.

Insect biochemistry and molecular biology·2026
Same author

NMR resonance assignments (¹H, ¹³C, ¹⁵N) of jararacin (rJarc), a disintegrin from Bothrops jararaca.

Biomolecular NMR assignments·2026
Same author

Engineering stable and reusable biocatalysts through electrostatic immobilization of (S)-norcoclaurine synthase on mesostructured kaolin.

International journal of biological macromolecules·2026
Same author

Engineering a Stable Grb2 Monomer: The W60A Mutation Disrupts Dimerization but Preserves Structural Integrity.

ACS omega·2026
Same author

Multi-biofluid metabolomics coupled with gene network reveals stage-specific alterations in mild cognitive impairment and Alzheimer's disease in an ethnically mixed cohort.

Brain research·2026
Same author

Endosymbiosis in trypanosomatids: the bacterium regulates the intermediate and oxidative metabolism of the host cell.

mSphere·2025

Area of Science:

  • Proteomics
  • Structural Biology
  • Biochemistry

Background:

  • Xanthomonas axonopodis pv citri is a plant pathogen requiring further study.
  • Structural proteomics aims to understand protein structures for functional insights.
  • High-throughput screening is crucial for efficient target identification.

Purpose of the Study:

  • To develop and apply a high-throughput strategy for screening protein targets in Xanthomonas axonopodis pv citri.
  • To classify soluble protein candidates based on their foldedness using NMR analysis.
  • To establish a priority list for selecting proteins for further structural investigations.

Main Methods:

  • Application of a high-throughput strategy for screening protein targets.
  • Utilizing rapid (1)H-(15)N HSQC Nuclear Magnetic Resonance (NMR) analysis.

Related Experiment Videos

  • Analysis of bacterial lysates containing selectively (15)N-labelled heterologous proteins.

    • Main Results:

    • Successfully classified 19 soluble protein candidates based on their foldedness.
    • NMR spectral quality directly correlated with the extent of well-folded solution structure.
    • Generated a priority list to guide the selection of protein candidates for structural studies.

    Conclusions:

    • The developed high-throughput NMR-based strategy is effective for assessing protein foldedness.
    • This method facilitates efficient prioritization of protein targets for structural proteomics.
    • The findings provide a valuable guide for future structural studies of Xanthomonas axonopodis pv citri proteins.