Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Frameshift mutation events in beta-glucosidases.

Antonio Rojas1, Santiago Garcia-Vallvé, Miguel A Montero

  • 1Evolutionary Genomics Group, Department of Biochemistry and Biotechnology, Rovira i Virgili University, Pl. Imperial Tàrraco, 1. E-43005, Catalonia, Tarragona, Spain.

Gene
|October 7, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Vitamin D in the prevention of left ventricular remodelling after an acute myocardial infarction: a randomized clinical trial.

Scientific reports·2026
Same author

PDB-CAT: A user-friendly tool to classify and analyze PDB protein-ligand complexes.

Protein science : a publication of the Protein Society·2025
Same author

Impact of the natural female reproductive aging on the rat serum lipidome.

Clinical science (London, England : 1979)·2025
Same author

Toward a Self-Inflating Bag Mechanical Ventilator, Maturation in Continuous Use, Safety, and Repeatability for Ventilatory Variables.

Journal of investigative surgery : the official journal of the Academy of Surgical Research·2025
Same author

Molecular Imprints of Clinical Comorbidities in Hypothalamic Extracellular Vesicles at the Onset of Obesity.

ACS omega·2025
Same author

Docosahexaenoic Acid (DHA) Supplementation in a Triglyceride Form Prevents from Polyglutamine-Induced Dysfunctions in <i>Caenorhabditis elegans</i>.

International journal of molecular sciences·2024

Compensated frameshift mutations are widespread genetic events impacting protein sequences. Computational analysis revealed these mutations in beta-glucosidase families, improving sequence alignments after neutralization.

Area of Science:

  • Genetics
  • Molecular Biology
  • Bioinformatics

Background:

  • Compensated frameshift mutations alter gene reading frames through various molecular events.
  • These mutations are often detected by comparing homologous amino acid and nucleotide sequences.
  • Identifying these events requires analyzing sequence conservation patterns.

Purpose of the Study:

  • To identify compensated frameshift mutations within O-glycoside hydrolase families 1 and 3.
  • To computationally neutralize identified frameshift events and assess their impact on sequence alignment.
  • To explore the structural implications of compensated frameshift mutations.

Main Methods:

  • Sequence analysis focused on identifying regions with lower protein conservation but conserved DNA in beta-glucosidase families.

Related Experiment Videos

  • Study restricted to seed sequences from O-glycoside hydrolase families 1 and 3.
  • Computational neutralization of frameshift events by removing insertions and replacing deletions with 'N'.

    • Main Results:

    • Compensated frameshift mutations were identified in beta-glucosidase family 1 (Erwinia herbicola, Cellulomonas fimi, Trifolium repens) and family 3 (Clostridium thermocellum, Clostridium stercorarium).
    • Computational neutralization of frameshifting subsequences improved beta-glucosidase alignments.
    • The study discusses the structural consequences of these mutations.

    Conclusions:

    • Compensated frameshift mutations are significant genetic phenomena observable in specific beta-glucosidase families.
    • Computational methods can effectively neutralize these mutations, leading to improved sequence alignments.
    • Further investigation into the structural and functional impacts of these mutations is warranted.