Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

HaCaT cell proliferation influenced by melatonin.

U-C Hipler1, T W Fischer, P Elsner

  • 1Department of Dermatology and Allergology, Friedrich-Schiller-Universität Jena, Jena, Germany. Christina.Hipler@med.uni-jena.de

Skin Pharmacology and Applied Skin Physiology
|October 7, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[New specialist training regulations in dermatology: Old wine in new bottles?]

Der Hautarzt; Zeitschrift fur Dermatologie, Venerologie, und verwandte Gebiete·2021
Same author

Validation of questionnaire algorithm based on repeated open application testing with the constituents of fragrance mix II: the EDEN Fragrance Study.

Journal of the European Academy of Dermatology and Venereology : JEADV·2021
Same author

[Pre- and post-interventional skin care for laser and peel treatments].

Der Hautarzt; Zeitschrift fur Dermatologie, Venerologie, und verwandte Gebiete·2021
Same author

[Phototherapy in the "City of Light" Jena].

Der Hautarzt; Zeitschrift fur Dermatologie, Venerologie, und verwandte Gebiete·2020
Same author

Basal cell carcinoma risk and solar UV exposure in occupationally relevant anatomic sites: do histological subtype, tumor localization and Fitzpatrick phototype play a role? A population-based case-control study.

Journal of occupational medicine and toxicology (London, England)·2020
Same author

[Dermatology in the medical services of the German Armed Forces-an essential medical specialty].

Der Hautarzt; Zeitschrift fur Dermatologie, Venerologie, und verwandte Gebiete·2020
Same journal

Epinastine inhibits eosinophil chemotaxis and adhesion molecules in atopic dermatitis.

Skin pharmacology and applied skin physiology·2003
Same journal

Role of isopropyl myristate, isopropyl alcohol and a combination of both in hydrocortisone permeation across the human stratum corneum.

Skin pharmacology and applied skin physiology·2003
Same journal

N-acetyltransferase 2 acetylation polymorphism: prevalence of slow acetylators does not differ between atopic dermatitis patients and healthy subjects.

Skin pharmacology and applied skin physiology·2003
Same journal

Detection of enhanced monohydroxyeicosatetraenoic acid and F2-isoprostane levels in human plasma samples after extracorporeal photoimmunotherapy.

Skin pharmacology and applied skin physiology·2003
Same journal

Hydrating effects of a corticoid oil formulation and its vehicle on human skin.

Skin pharmacology and applied skin physiology·2003
Same journal

Testosterone metabolism in human skin cells in vitro and its interaction with estradiol and dutasteride.

Skin pharmacology and applied skin physiology·2003
See all related articles

Melatonin influences skin cell proliferation, with its effects varying by concentration and incubation time. This study determined safe melatonin levels for skin treatments, avoiding cytotoxicity in HaCaT keratinocytes.

Area of Science:

  • Pharmacology
  • Dermatology
  • Cell Biology

Background:

  • Melatonin is a hormone with diverse pharmacological effects.
  • Previous research indicates melatonin influences human hair follicle growth.

Purpose of the Study:

  • To investigate the effects of melatonin on HaCaT keratinocyte proliferation.
  • To determine safe melatonin concentrations for skin treatments.
  • To assess potential cytotoxic effects of melatonin on skin cells.

Main Methods:

  • Cell proliferation was measured using [3H]thymidine incorporation.
  • A fluorescence assay with Hoechst dye 33342 was employed.
  • ATP bioluminescence assays were conducted.

Main Results:

Related Experiment Videos

  • Melatonin demonstrated varying proliferative activity on HaCaT keratinocytes.
  • The observed effects were dependent on melatonin concentration and incubation time.
  • No cytotoxic effects were observed within the tested concentration ranges.

Conclusions:

  • Melatonin can modulate skin cell proliferation in a dose- and time-dependent manner.
  • Specific melatonin concentrations are suitable for skin treatments without causing cytotoxicity.
  • Further research into melatonin's dermatological applications is warranted.