Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Neuron cell type-specific SNAP-25 expression driven by multiple regulatory elements in the nematode Caenorhabditis

Soon Baek Hwang1, Junho Lee

  • 1National Research Laboratory, Department of Biology, Yonsei University, 134 Shinchon-dong, Seodaemun-ku, Seoul 120-749, South Korea.

Journal of Molecular Biology
|October 8, 2003
PubMed
Summary

Researchers identified regulatory elements controlling SNAP-25 gene expression in neurons. Specific elements dictate expression in distinct neuronal types, involving transcription factors like UNC-86 and MEC-3.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Scalp salvage with acellular dermal matrix assistance for recurrent exposure of deep brain stimulation hardware: a case report.

Archives of craniofacial surgery·2026
Same author

HIV-1 protease cleavage sites detection with a quantum convolutional neural network algorithm.

Scientific reports·2026
Same author

Comparative connectomics reveals stage-specific gap junction rewiring that reshapes avoidance behavior.

bioRxiv : the preprint server for biology·2026
Same author

Confidence Set for the Cluster of a Spatial Scan Statistic.

Statistics in medicine·2026
Same author

Association Between Illness Severity Scores and Quantitatively Measured Brain Injury in Cardiac Arrest Survivors.

Journal of clinical medicine·2026
Same author

Prediction of intrinsic solubility for drug-like organic compounds using automated network optimizer (ANO) for physicochemical feature and hyperparameter optimization.

Journal of cheminformatics·2026

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genetics

Background:

  • The SNAP-25 gene is crucial for neuronal function and synaptic vesicle exocytosis.
  • Understanding its regulation is key to deciphering neuronal development and activity.

Purpose of the Study:

  • To identify and characterize the regulatory elements governing neuronal SNAP-25 gene expression in nematodes.
  • To elucidate the specific roles of these elements in different neuronal populations.

Main Methods:

  • Comparative sequence analysis of SNAP-25 genes in Caenorhabditis elegans and Caenorhabditis briggsae.
  • Reporter assays to test the function of putative regulatory elements (P1, P2, I1h, I1m, I2h, I2m).
  • Nuclear extract binding assays and genetic mutations to identify involved transcription factors (UNC-86, MEC-3).

Related Experiment Videos

Main Results:

  • Identified two 5' regulatory elements (P1, P2) and four intronic elements (I1h, I1m, I2h, I2m).
  • Demonstrated cell-type-specific requirements for these elements in various neurons (DA, DD, DB, amphid, phasmid, pharyngeal, touch receptor).
  • Showed that the I2h element requires transcription factors UNC-86 and MEC-3 for activity in mechanosensory neurons.

Conclusions:

  • Neuronal SNAP-25 expression is regulated by a complex interplay of distinct cis-regulatory elements.
  • Specific transcription factors, including UNC-86 and MEC-3, bind to and regulate these elements in a cell-specific manner.
  • Transcriptional regulation of SNAP-25 involves multiple factors, highlighting intricate control mechanisms in neuronal gene expression.