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Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization
Published on: April 14, 2016
An efficient strategy for screening large cloned libraries of amplified 16S rDNA sequences from complex environmental
J M Gonzalez1, A Ortiz-Martinez, M A Gonzalez-delValle
1Instituto de Recursos Naturales y Agrobiologia, Consejo Superior de Investigaciones Científicas, P.O. Box 1052, 41080, Seville, Spain. jmgrau@irnase.csic.es
Journal of Microbiological Methods
|October 8, 2003
Abstract:
We propose a strategy for the efficient screening of large libraries of amplified 16S rRNA genes from complex environmental samples. It consists of processing sets of multiple clones simultaneously. This strategy saves up to 90% of the costs and labor spent in the process of screening a 16S rDNA library.

