Sulfatides: targets for anti-phospholipid antibodies
M Merten1, S Motamedy, S Ramamurthy
1Departments of Pathology and Medicine, Baylor College of Medicine, Houston, Tex, USA.
Beta2-glycoprotein I binds to sulfatides, suggesting these molecules are targets for anti-phospholipid antibodies. This interaction may explain clinical symptoms associated with anti-phospholipid antibody syndrome.
Area of Science:
- Immunology
- Glycobiology
- Hematology
Background:
- Sulfatides are glycosphingolipids on blood cell surfaces involved in hemostasis.
- Beta2-glycoprotein I binds anionic phospholipids and is targeted by anti-phospholipid antibodies, linked to thrombosis and miscarriage.
Purpose of the Study:
- To investigate if beta2-glycoprotein I forms complexes with sulfatides.
- To determine if these complexes are targets for anti-phospholipid antibodies.
Main Methods:
- ELISA assays to measure binding of beta2-glycoprotein I to sulfatides.
- Testing patient sera containing anti-cardiolipin antibodies for reactivity with sulfatide-bound beta2-glycoprotein I.
Main Results:
- Beta2-glycoprotein I specifically binds to surface-bound sulfatides, not other glycolipids.
- Patient sera with anti-cardiolipin antibodies showed reactivity with the sulfatide-beta2-glycoprotein I complex.
- Purified anti-cardiolipin antibodies also reacted with the complex.
Conclusions:
- Sulfatides, in addition to anionic phospholipids, are targets for anti-phospholipid antibodies.
- Antibody interactions with sulfatides may contribute to anti-phospholipid antibody syndrome symptoms.
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