Gene expression changes in the human fibroblast induced by Centella asiatica triterpenoids

Area of Science:

• Molecular Biology
• Pharmacology
• Dermatology

Background:

• Centella asiatica is a tropical plant used in traditional medicine.
• Triterpenoid compounds from Centella asiatica possess diverse biological activities.
• Understanding the molecular mechanisms of these compounds is crucial for therapeutic applications.

Purpose of the Study:

• To investigate the molecular pathways of Centella asiatica triterpenoids.
• To evaluate the effect of Centella asiatica extract and its components on gene expression in human fibroblasts.
• To correlate gene expression changes with the known therapeutic uses of Centella asiatica.

Main Methods:

• Gene microarrays and real-time reverse transcription polymerase chain reaction (real-time RT-PCR) were employed.
• Quantification of 1053 human gene expressions in cultured human fibroblasts.
• Treatment with titrated extract from Centella asiatica (TECA) and its four principal triterpenoid components.

Main Results:

• TECA affects genes involved in angiogenesis and extracellular matrix remodeling.
• Gene expression changes, particularly for TNFAIP6, were dose-dependent and time-dependent (48 hours).
• Asiatic acid and madecassic acid showed the strongest response for TNFAIP6 expression.

Conclusions:

• Centella triterpenes induce gene expression patterns consistent with wound healing and connective tissue disorder treatments.
• Identified modulated genes provide a molecular basis for Centella's bioactivity.
• This research offers opportunities for correlating molecular activity with clinical effectiveness.