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A catalogue of gene expression in the developing kidney
Kristopher Schwab1, Larry T Patterson, Bruce J Aronow
1Division of Developmental Biology, Division of Nephrology, Children's Hospital Research Foundation, Children's Hospital Medical Center, Cincinnati, Ohio 45229, USA.
Kidney International
|October 9, 2003
Summary
This study used microarrays to analyze gene expression in developing mouse kidneys, identifying thousands of novel genes involved in kidney organogenesis. The findings establish a baseline for future research on kidney development and related genetic mutations.
Area of Science:
- Developmental Biology
- Genomics
- Molecular Biology
Background:
- Kidney morphogenesis involves numerous genes, with many yet to be identified.
- Microarray technology enables global analysis of gene expression in biological development.
Purpose of the Study:
- To define gene expression profiles of the developing mouse kidney using microarrays.
- To identify novel genes associated with kidney organogenesis.
Main Methods:
- Utilized Affymetrix U74Av2 microarrays (over 12,000 genes) with microamplification.
- Analyzed gene expression in microdissected murine ureteric bud, metanephric mesenchyme, and whole kidneys at various embryonic and adult stages.
- Employed in situ hybridizations for validation.
Main Results:
- Identified 3,847 genes in the E12.5 embryonic kidney.
- Discovered 428 genes with significantly elevated expression in the embryonic kidney compared to adult kidneys.
- Found limited gene expression differences between ureteric bud and metanephric mesenchyme.
- Observed similar expression patterns for Cdrap, Tgfbi, and Col15a1 in developing kidneys and lungs.
Conclusions:
- Successfully identified numerous genes not previously linked to kidney development.
- Established a comprehensive gene expression catalogue for the developing mouse kidney and its components.
- Provided a baseline for future studies using mouse models of kidney development and disease.