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Coaggregation among nonflocculating bacteria isolated from activated sludge.

Anushree Malik¹, Masashi Sakamoto, Shohei Hanazaki

¹Department of Applied Chemistry, Faculty of Engineering, Utsunomiya University, Utsunomiya 321-8585, Japan.

Applied and Environmental Microbiology

|October 9, 2003

Summary

Nonflocculating bacteria from activated sludge can coaggregate, forming flocs. Acinetobacter johnsonii S35 demonstrated significant coaggregation with multiple bacterial genera, highlighting their role in sludge flocculation.

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Area of Science:

Environmental microbiology
Wastewater treatment microbiology

Background:

Activated sludge flocs are crucial for wastewater treatment efficiency.
The role of nonflocculating bacteria in floc formation is often overlooked.

Purpose of the Study:

To investigate the coaggregation potential of nonflocculating bacteria from sewage-activated sludge.
To identify bacterial strains capable of coaggregation and their interactions with Acinetobacter species.

Main Methods:

Spectrophotometric assay to quantify coaggregation in two-membered bacterial systems.
Identification of bacterial strains using full-length 16S rRNA gene sequencing.

Main Results:

Eight of 32 nonflocculating strains showed significant coaggregation (74-99%) with Acinetobacter johnsonii S35.

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Four strains exhibited moderate coaggregation (43-65%) with Acinetobacter junii S33.

Coaggregation patterns correlated with cellular characteristics and aggregate size.

A. johnsonii S35 coaggregated with diverse genera, including Oligotropha, Microbacterium, and Xanthomonas.

Conclusions:

Nonflocculating bacteria play a significant role in the formation of activated sludge flocs.
Acinetobacter isolates can act as bridging organisms in multigeneric coaggregates.
This study reveals the importance of previously neglected bacteria in sludge granulation and wastewater treatment processes.