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Updated: Aug 30, 2026
Establishing Stable Binary Cultures of Symbiotic Saccharibacteria from the Oral Cavity
Published on: April 13, 2021
Single-cell enumeration of an uncultivated TM7 subgroup in the human subgingival crevice
Cleber C Ouverney1, Gary C Armitage, David A Relman
1Department of Microbiology and Immunology, Stanford University, Stanford, California 94305, USA. ouverney@stanford.edu
Abstract:
Specific oligonucleotide hybridization conditions were established for single-cell enumeration of uncultivated TM7 and IO25 bacteria by using clones expressing heterologous 16S rRNA. In situ analysis of human subgingival crevice specimens revealed that a greater proportion of samples from sites of chronic periodontitis than from healthy sites contained TM7 subgroup IO25. In addition, IO25 bacterial cells from periodontitis site samples were more abundant and fourfold longer than IO25 cells from healthy site samples.
