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Updated: Aug 30, 2026

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing
Published on: July 7, 2020
Development of a mariner-based transposon for use in Sorangium cellulosum
1Kosan Biosciences, Inc., Hayward, California 94545, USA. julien@kosan.com
Abstract:
In order to generate marked insertions in the myxobacterium Sorangium cellulosum, a transposon based on the eukaryotic mariner transposon was developed. The transposition frequency was increased with the use of a mutated tnp gene. The transposon randomly inserts into the chromosome, as demonstrated by targeted mutagenesis of the epoK gene.
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