Related Experiment Video
Updated: Aug 30, 2026

Quantifying the Antifungal Activity of Peptides Against Candida albicans
Published on: January 13, 2023
Differentiation of Candida dubliniensis from Candida albicans on Pal's agar
Asmaa Al Mosaid1, Derek J Sullivan, David C Coleman
1Microbiology Research Unit, Department of Oral Medicine and Oral Pathology, School of Dental Science and Dublin Dental Hospital, Trinity College, University of Dublin, Dublin 2, Republic of Ireland.
Abstract:
Production of a hyphal fringe around colonies grown on Pal's agar (sunflower seed agar) at 30 degrees C for 48 to 72 h provides a simple means of discriminating between isolates of C. dubliniensis and C. albicans with 100% accuracy. Of 128 C. dubliniensis isolates tested on this medium, all produced a hyphal fringe. In contrast, none of the 124 C. albicans isolates tested produced a hyphal fringe. Pal's medium has the added advantage of being prepared from inexpensive, readily available seeds.

