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Evaluation of a multilocus sequence typing system for Staphylococcus epidermidis.

Xin-Min Wang1, Liliane Noble1, Barry N Kreiswirth1

  • 1Merck and Co. Inc., PO Box 4 WP 26-265, Sumneytown Pike, West Point, PA 19486, USA 2Merck and Co. Inc., PO Box 2000 RY80Y 230, 126 E. Lincoln Ave., Rahway, NJ 07065, USA 3PHRI TB Center, Public Health Research Institute, 225 Warren Street, Newark, NJ 07103, USA.

Journal of Medical Microbiology
|October 9, 2003
PubMed
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Multilocus sequence typing (MLST) effectively groups Staphylococcus epidermidis strains at the subspecies level, aiding in identifying this common cause of hospital-acquired infections. However, it is less discriminatory than other methods for detailed epidemiological studies.

Area of Science:

  • Microbiology
  • Genetics
  • Epidemiology

Background:

  • Staphylococcus epidermidis is a major cause of nosocomial infections.
  • The subspecies-level taxonomy of S. epidermidis is not well-defined.

Purpose of the Study:

  • To investigate a multilocus sequence typing (MLST) scheme for clarifying taxonomic relationships within S. epidermidis.
  • To assess the discriminatory power of MLST compared to PFGE for S. epidermidis strain typing.

Main Methods:

  • DNA sequences of seven housekeeping genes were analyzed from 47 clinical S. epidermidis isolates.
  • Multilocus sequence typing (MLST) was employed to determine allelic profiles.
  • Pulsed-field gel electrophoresis (PFGE) of SmaI-digested genomic DNA was used for comparative analysis.

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Main Results:

  • Twenty-three distinct allelic profiles were identified by MLST.
  • PFGE resolved isolates within the largest MLST profile group into four distinct clusters.
  • MLST confirmed the identity of coagulase-negative staphylococci (CoNS) isolates.

Conclusions:

  • MLST is effective for subspecies-level grouping of S. epidermidis strains.
  • MLST alone lacks the discriminatory power for detailed epidemiological investigations.
  • MLST is a valuable tool for confirming S. epidermidis identification.