Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Chemotherapy increases transgene expression in leukemic cells.

Rodolphe Vereecque1, Aurore Saudemont1, Stéphane Depil1,2

  • 1Unité INSERM 524, Institut de Médecine Prédictive et de Recherche Thérapeutique, Lille (IFR-114), France.

The Journal of Gene Medicine
|October 9, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chlorophenylpyrazolone derivatives as novel PD-L1-targeted inhibitors of the PD-1/PD-L1 immune checkpoint.

Bioorganic & medicinal chemistry letters·2026
Same author

Selective access to individual cells after assembling for advanced cell-cell interaction studies.

Journal of nanobiotechnology·2026
Same author

Mapping National Definitions, Classifications, and Policy Approaches to Poor-Prognosis Cancers Across the G7 Cancer Initiative Countries.

JCO global oncology·2026
Same author

Pre-therapeutic bone marrow-resident leukemic cells in acute myeloid leukemia exhibit a distinct dysregulated calcium signature and stem-like profile reflecting minimal residual disease precursors.

Journal of experimental & clinical cancer research : CR·2026
Same author

Call for preserving specialized knowledge and contributions of the CAT to advancing ATMPs in Europe.

Cytotherapy·2025
Same author

Orthogonal IMiD-Degron Pairs Induce Selective Protein Degradation in Cells.

ACS chemical biology·2025

Chemotherapy drugs like daunorubicin and cytosine arabinoside can boost gene therapy effectiveness in acute myeloid leukemia (AML) cells. This finding supports combining chemotherapy with gene therapy for improved AML treatment outcomes.

Area of Science:

  • Oncology
  • Molecular Biology
  • Gene Therapy

Background:

  • Acute myeloid leukemia (AML) frequently relapses after chemotherapy.
  • Gene therapy offers a promising adjunct to chemotherapy for AML.
  • The impact of chemotherapy on transgene expression in leukemia cells remains unexplored.

Purpose of the Study:

  • To investigate the effect of chemotherapy on transgene expression in leukemic cells.
  • To evaluate the potential of combining chemotherapy with gene therapy for AML treatment.

Main Methods:

  • Transfection of DA1-3b leukemic cells with GM-CSF or LacZ plasmids.
  • Transduction of K562 and primary AML cells with an adenoviral vector (Ad5.CMV-LacZ).
  • Incubation with daunorubicin (DNR) and cytosine arabinoside (Ara-C), followed by transgene expression analysis.

Related Experiment Videos

Main Results:

  • DNR and Ara-C significantly increased LacZ and GM-CSF transgene expression in a dose-dependent manner.
  • Chemotherapy-induced overexpression was linked to increased transcription and reactive oxygen species.
  • Enhanced transgene expression was observed in K562 cells, primary AML samples, and in vivo mouse models.

Conclusions:

  • Chemotherapeutic agents can induce transgene overexpression in leukemic cells.
  • This synergistic effect presents a promising strategy for combined chemotherapy and gene therapy in acute leukemia treatment.