Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Lectin cross-linking interactions with multivalent carbohydrates.

C F Brewer1

  • 1Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York 10641, USA.

Advances in Experimental Medicine and Biology
|October 10, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Nerve block efficacy in breast augmentation: A systematic review and meta-analysis.

Journal of plastic, reconstructive & aesthetic surgery : JPRAS·2023
Same author

Platelet-Rich Plasma (PRP) for Sacrococcygeal Pilonidal Disease: An Updated Systematic Review and Meta-Analysis.

World journal of surgery·2022
Same author

Chyle leak following total colectomy for ulcerative colitis: a case report and review of the literature.

Annals of the Royal College of Surgeons of England·2021
Same author

Purple Urine Bag Syndrome in a Patient with an Ileal Conduit and Clostridium Difficile Infection.

Acute medicine·2020
Same author

Phosphorylated human lectin galectin-3: analysis of ligand binding by histochemical monitoring of normal/malignant squamous epithelia and by isothermal titration calorimetry.

Anatomia, histologia, embryologia·2008
Same author

Directing the immune response to carbohydrate antigens.

The Journal of biological chemistry·2001
Same journal

Peptidomics in the Spotlight: Advanced Sample Treatment Techniques and Analytical Insights.

Advances in experimental medicine and biology·2026
Same journal

Methods for the Investigation of Protein-Ligands Interactions.

Advances in experimental medicine and biology·2026
Same journal

Sample Preparation Strategies for Microbial Cell Surface Proteomics: Integrating Shaving and Shotgun Approaches.

Advances in experimental medicine and biology·2026
Same journal

Proteomic Sample Preparation for the Petroleum Industry: A Biocorrosion Case Study.

Advances in experimental medicine and biology·2026
Same journal

Proteomic and Functional Comparison of Extracellular Vesicles from Wild-Type and Lyn-Deficient Stromal Cells.

Advances in experimental medicine and biology·2026
Same journal

Proteomic Analysis of Histone Sequence Variants and Post-translationally Modified Forms.

Advances in experimental medicine and biology·2026
See all related articles

This study reveals how multivalent carbohydrate-lectin interactions form specific, cross-linked lattices. X-ray data show distinct complexes, providing a molecular basis for cellular recognition and signal transduction.

Area of Science:

  • Structural Biology
  • Biochemistry
  • Glycobiology

Background:

  • Multivalent carbohydrate-lectin interactions are crucial for cellular recognition and signal transduction.
  • Previous understanding of specificity in these interactions was limited.
  • Lectin-carbohydrate cross-linking is implicated in cellular processes like T-cell apoptosis.

Purpose of the Study:

  • To provide a molecular basis for a new paradigm of specificity in multivalent carbohydrate-lectin interactions.
  • To elucidate the formation of type 2 homogeneous cross-linked lattices.
  • To model lectin-carbohydrate clustering in vivo using crystal structures.

Main Methods:

  • X-ray crystallography was used to determine the structures of cross-linked complexes.
  • Analysis of complexes formed between structurally related divalent carbohydrates and a tetravalent lectin (SBA).

Related Experiment Videos

  • Comparison with confocal microscopy data showing homogeneous aggregates of cross-linked receptors.

    • Main Results:

    • Distinct cross-linked complexes were observed between divalent carbohydrates and the tetravalent lectin (SBA), influenced by crystal packing.
    • A molecular basis for the formation of homogeneous type 2 cross-linked complexes was established.
    • The crystal structures serve as models for in vivo lectin-carbohydrate clustering.

    Conclusions:

    • The findings establish a new paradigm for specificity in multivalent carbohydrate-lectin interactions via homogeneous type 2 cross-linked lattices.
    • These interactions are fundamental to cellular recognition and signal transduction pathways.
    • The study provides structural insights relevant to biological processes involving lectin-glycoprotein interactions.