[Study of ceratocystis fimbriata toxins determination from sweet potato by high performance thin-layer...

The extract solution from Sweet Potato was washed with 5% Na2CO3 and followed with distilled water and dried with dehydrated sodium sulfate (Na2SO4). The ether extract was evaporated and the residue (crude sample) was dissolved in a small volume of chloroform-soluble solution and was spotted on a silica gel G thin-layer plate developed with different solvent systems. Pure ipomeamarone and ipomeamaronol were used as standards. The resolution was the best in the solvent system of petroleum ether-ethylacetate at 20 degrees C. The separated spots were scanned by CS-930 TLC scanner at 520 nm and quantitative determination by external standard calibration. Ipomeamarone was detected by high performance thin-layer chromatography and the limits of detection were 0.002 microgram for pure and 0.01 microgram for crude sample.