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Published on: October 23, 2011
A novel multiplex PCR system consisting of Y-STRs DYS441, DYS442, DYS443, DYS444, and DYS445
Reiko Iida1, Kazumi Sawazaki, Hitoe Ikeda
1Department of Forensic Medicine, Fukui Medical University, Fukui 910-1193, Japan. ireiko@fmsrsa.fukui-med.ac.jp
Abstract:
We have developed a new sensitive multiplex PCR system consisting of five male-specific and polymorphic tetranucleotide STRs--DYS441 (GDB: 10013873), DYS442 (GDB: 10030304), DYS443 (GDB: 10807127), DYS444 (GDB: 10807128), and DYS445 (GDB: 10807129) on the Y chromosome. Fifty pg DNA per 10 microL reaction volume was required for the correct typing of five STRs. Using this system, the five Y-STRs were correctly typed from blood and semen stains that had been stored for several years at room temperature.
