Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ephective endocytosis.

Michael M Halford1, Michael J Chumley, Mark Henkemeyer

  • 1Center for Developmental Biology, University of Texas Southwestern Medical Center, 6000 Harry Hines Boulevard, Dallas, TX 75390, USA.

Developmental Cell
|October 11, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multiplex In Vivo Imaging of Biodistribution and Pharmacokinetics of CoMoCAT Single-Walled Carbon Nanotubes.

ACS applied bio materials·2026
Same author

Eph-Ephrin Tetramerization Inhibitors Target Bidirectional Signaling to Combat Pain and Addiction.

bioRxiv : the preprint server for biology·2025
Same author

Proteinaceous Metal-Binding Eph-Ephrin Tetramerization is Modulated by Copper and Chelators.

bioRxiv : the preprint server for biology·2025
Same author

Diet-induced MASH liver fibrosis promoted by EphB2 can be targeted by small molecule tetramerization inhibitors.

bioRxiv : the preprint server for biology·2025
Same author

An endothelial cell competition assay for determinants of the response to targeted anti-angiogenics.

Growth factors (Chur, Switzerland)·2025
Same author

A Mediterranean-style diet protects against cognitive and behavioral deficits, adiposity, and Alzheimer's disease-related markers, compared to a macronutrient-matched typical American diet in C57BL/6J mice.

Journal of Alzheimer's disease : JAD·2025

Endocytosis of EphB-ephrinB complexes switches cell adhesion to repulsion. This, with ephrinA shedding, explains how adhesive interactions trigger repulsive cellular responses.

Area of Science:

  • Cellular biology
  • Molecular biology
  • Developmental biology

Background:

  • Cell-cell interactions are crucial for development and tissue homeostasis.
  • Receptor-ligand interactions mediate diverse cellular responses, including adhesion and repulsion.
  • The Eph/ephrin signaling system plays key roles in guiding cell movements and tissue patterning.

Purpose of the Study:

  • To elucidate the molecular mechanisms underlying the switch between cell adhesion and repulsion.
  • To understand how Eph/ephrin signaling contributes to bidirectional cell communication.
  • To resolve the paradox of adhesive interactions leading to repulsive cellular outcomes.

Main Methods:

  • Investigated the role of endocytosis in EphB-ephrinB complex regulation.

Related Experiment Videos

  • Analyzed EphA-ephrinA interactions and their downstream effects.
  • Utilized advanced microscopy and biochemical assays to track receptor-ligand dynamics.

    • Main Results:

    • Identified endocytosis of EphB-ephrinB complexes as a critical mechanism for switching cell behavior.
    • Demonstrated that EphA-ephrinA shedding also contributes to repulsive signaling.
    • Showcased how adhesive receptor-ligand engagement can generate repulsive cellular responses.

    Conclusions:

    • Endocytosis of EphB-ephrinB complexes is a key regulator of cell-cell adhesion and repulsion.
    • Combined with ephrinA shedding, these mechanisms provide a comprehensive model for Eph/ephrin-mediated cell guidance.
    • These findings resolve a long-standing paradox in cell signaling research.