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Escherichia coli: rapid identification by chromogenic tests.

S F Dealler1, D Law, J Collins

  • 1University of Leeds, Microbiology Department, UK.

Medical Laboratory Sciences
|March 1, 1992
PubMed
Summary

A rapid triple chromogenic test accurately identifies Escherichia coli (E. coli) by detecting specific enzyme activities. This cost-effective method demonstrates high specificity and sensitivity for reliable E. coli detection in clinical samples.

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Area of Science:

  • Microbiology
  • Clinical Diagnostics
  • Bacteriology

Background:

  • Accurate identification of Escherichia coli (E. coli) is crucial for patient treatment and infection control.
  • Traditional methods for E. coli identification can be time-consuming and resource-intensive.

Purpose of the Study:

  • To evaluate a rapid triple chromogenic test for the identification of E. coli.
  • To assess the specificity and sensitivity of this chromogenic test compared to established methods.

Main Methods:

  • A triple chromogenic test detecting beta-galactosidase, beta-glucuronidase, and indole production was used.
  • The test was applied to 514 confirmed E. coli strains and 512 non-E. coli strains.
  • Further validation was performed on 221 coliforms from blood cultures, comparing results with the API 20E system.

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Main Results:

  • The chromogenic test showed 94.5% sensitivity and 100% specificity for E. coli identification among pure cultures.
  • In blood culture isolates, the test achieved 94% sensitivity and 100% specificity for E. coli.
  • No false positives were observed in the non-E. coli group.

Conclusions:

  • The rapid triple chromogenic test is a highly specific and sensitive method for E. coli identification.
  • The test is rapid, simple to perform, and economical, making it suitable for clinical laboratories.
  • This method offers a reliable alternative for timely E. coli detection.