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Related Experiment Videos

Serum low density lipoprotein separation: a simple procedure.

M S Graziani1, G Dimitri, U Lippi

  • 1Laboratorio di Chimica Clinica ed Ematologia, Ospedale Civile Maggiore, Verona, Italy.

Medical Laboratory Sciences
|March 1, 1992
PubMed
Summary
This summary is machine-generated.

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A new method provides native low density lipoprotein (LDL) for studies, avoiding time-consuming ultracentrifugation and preserving lipoprotein structure and function.

Area of Science:

  • Biochemistry
  • Lipidology

Background:

  • Standard low density lipoprotein (LDL) separation methods are often time-consuming.
  • Ultracentrifugation using salt gradients can alter lipoprotein structure and immunochemistry.

Purpose of the Study:

  • To develop a rapid and effective method for isolating native LDL.
  • To ensure the isolated LDL is suitable for chemical and immunochemical analyses.

Main Methods:

  • A three-step procedure involving ultracentrifugation, polyethylene glycol precipitation, and salt re-dissolution.
  • Agarose gel electrophoresis to assess LDL separation and electrophoretic mobility.
  • Immunochemical assays to verify apolipoprotein B reactivity.

Main Results:

  • Successfully separated LDL from other lipoproteins.

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  • Preserved the electrophoretic mobility of LDL molecules.
  • Maintained the immunochemical reactivity of apolipoprotein B.

    • Conclusions:

    • The developed method efficiently isolates native LDL.
    • This technique is suitable for subsequent chemical and immunochemical studies.
    • It offers an alternative to traditional, potentially damaging separation methods.