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Expression vehicles used in recombinant DNA technology.

J D Friesen1, G An

  • 1Department of Medical Genetics. Medical Sciences Building. University of Toronto, Ontario, Canada.

Biotechnology Advances
|January 1, 1983
PubMed
Summary
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This review surveys gene cloning vehicles for Escherichia coli, Saccharomyces cerevisiae, and mammalian cells. It details various expression systems and strategies for gene product extrusion and replication.

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Genetics

Background:

  • Gene cloning and expression are fundamental techniques in molecular biology.
  • Efficient gene delivery and expression in various host systems are crucial for research and biotechnology.

Purpose of the Study:

  • To provide a comprehensive survey of cloning vehicles used for gene expression in diverse host cells.
  • To discuss specific examples of cloning vectors and strategies in E. coli, S. cerevisiae, and mammalian cells.

Main Methods:

  • Literature review of cloning vehicles and expression systems.
  • Categorization of vectors based on host organisms (E. coli, S. cerevisiae, mammalian cells).
  • Discussion of promoter elements, replication strategies, and gene product extrusion.

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Main Results:

  • Detailed overview of cloning vehicles utilizing operons (lac, trp, rho, recA) and open reading frames in E. coli.
  • Description of yeast vectors based on PGK, ADH1, acid phosphatase, and GAL1-GAL10 genes in S. cerevisiae.
  • Presentation of mammalian cell vectors employing SV40 promoters, metallothionein gene, retroviral LTRs, bovine papilloma virus, and vaccinia virus.

Conclusions:

  • A wide array of cloning vehicles are available for expressing genes in prokaryotic and eukaryotic systems.
  • Selection of appropriate cloning vehicles depends on the host organism and experimental objectives.
  • Strategies for enhanced gene expression and product recovery are critical for successful cloning.