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Updated: Aug 30, 2026

Generation of Murine Monoclonal Antibodies by Hybridoma Technology
Published on: January 2, 2017
Media for hybridoma growth and monoclonal antibody production
N C Bols1, J M Scharer, H A Phillips
1Department of Biology, University of Waterloo, Waterloo, Ontario, N2L 2G1, Canada.
Abstract:
For the economical production of monoclonal antibodies (MAbs), the cell-culture medium must be optimized for three different phases: growth of the hybridomas, MAb productivity of the hybridomas, and MAb purification or downstream processing. Medium improvements are necessary to meet these requirements for large-scale MAb production. Information bearing on this issue is being addressed in two research areas, cell biology and biochemical engineering, and is reviewed in this article.
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