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An overview of continuous protein purification processes.
N F Gordon1, C M Moore, C L Cooney
1Department of Chemical Engineering and Biotechnology Process Engineering Center, Massachusetts Institute of Technology, Cambridge 02139, USA.
Biotechnology Advances
|January 1, 1990
Summary
Continuous processing offers a promising approach to reduce costs in large-scale protein purification. This review examines adsorptive, electrophoretic, and extractive continuous methods for efficient manufacturing.
Area of Science:
- Biotechnology and biochemical engineering
- Process development and scale-up
- Recombinant protein manufacturing
Background:
- Recombinant technology's expansion necessitates scalable manufacturing processes.
- Protein purification costs significantly impact overall biopharmaceutical economics.
- Optimizing purification is critical for commercial success.
Purpose of the Study:
- To critically review the application of continuous processing in protein purification and recovery.
- To assess the feasibility of continuous methods for large-scale biomanufacturing.
- To identify operational advantages and limitations of continuous protein purification.
Main Methods:
- Review of continuous adsorptive and chromatographic processes.
- Examination of continuous electrophoretic techniques.
- Analysis of continuous extractive recovery methods.
Main Results:
- Continuous processing presents potential for cost reduction in protein purification.
- Adsorptive, electrophoretic, and extractive methods are key areas of continuous operation.
- Each continuous method exhibits specific operational advantages and limitations.
Conclusions:
- Continuous processing is a viable strategy for enhancing efficiency and reducing costs in large-scale protein purification.
- Further development and implementation of continuous methods are crucial for commercializing recombinant products.
- Understanding the trade-offs of different continuous techniques is essential for process optimization.