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Updated: Aug 30, 2026

Measuring Enzymatic Stability by Isothermal Titration Calorimetry
Published on: March 26, 2019
Influence of chemical modification on enzyme inactivation kinetics and stability
1Chemical Engineering Department, University of Mississippi, MS 38677, USA.
Abstract:
Enzymes are placed in different categories depending on the effect of chemical modification on their inactivation kinetics and residual activity. This is done using a series-type mechanism involving degraded but stable enzyme states. The major distinction in the three basic categories is the effect of modification on residual activity. Each category is further sub-divided depending on the effect of modification on the values of the deactivation rate constants. The classification provides for a framework for comparison of a wide variety of enzyme deactivation data. Structure-function relations are provided wherever possible.
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