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Related Experiment Videos

Downstream processing of microbial rennet from solid state fermented moldy bran.

M S Thakur1, N G Karanth, K Nand

  • 1Fermentation Technology and Bioengineering Discipline, Central Food Technological Research Institute, Mysore, India.

Biotechnology Advances
|January 1, 1993
PubMed
Summary

Microbial rennets from Mucor miehei offer a viable alternative to calf rennet. This study details an efficient method for producing powdered rennet with high enzymatic activity using clay filtration.

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Area of Science:

  • Biotechnology
  • Enzyme Technology
  • Food Science

Background:

  • Calf rennet shortage necessitates alternatives.
  • Microbial rennets are commercially viable.
  • Mucor miehei is a source of rennet.

Purpose of the Study:

  • To develop an efficient method for producing microbial rennet.
  • To purify rennet from Mucor miehei using clay filtration.
  • To obtain a stable, active powdered rennet product.

Main Methods:

  • Solid-state fermentation of Mucor miehei on bran.
  • Semicontinuous multiple contact forced percolation extraction.
  • Purification using 5% R16 clay filtration.
  • Enzyme precipitation with ethyl alcohol and drying.

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Main Results:

  • Efficient removal of lipase and protease impurities.
  • Production of powdered rennet without costly chemicals.
  • Achieved enzyme activity of 1.5 x 10(5) Soxhlet units/gm.

Conclusions:

  • Clay filtration is effective for purifying microbial rennet.
  • This method provides a cost-effective alternative to calf rennet.
  • The produced powdered rennet is stable and highly active.