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Related Experiment Videos

Development of high penicillin producing strains for solid state fermentation.

J Barrios-González1, T E Castillo, A Mejía

  • 1Depto. de Biotecnología, Universidad Autónoma Metropolitana, Iztapalapa, Apdo. Postal 55-535, 09340, México.

Biotechnology Advances
|January 1, 1993
PubMed
Summary

Penicillin production potential was evaluated in solid-state fermentation (SSF) versus liquid-submerged fermentation (LSF) using P. chrysogenum strains. High-yielding clones for SSF were identified, achieving significant production increases.

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Area of Science:

  • Biotechnology
  • Microbial Production
  • Fermentation Technology

Background:

  • Penicillin production is crucial in the pharmaceutical industry.
  • Comparing solid-state fermentation (SSF) and liquid-submerged fermentation (LSF) for antibiotic production is essential for optimizing yields.
  • Understanding strain-specific performance differences between SSF and LSF is key to strain improvement.

Purpose of the Study:

  • To determine penicillin production levels in industrial and P. chrysogenum strains using both SSF and LSF.
  • To evaluate the relative performance of strains and their clones in SSF compared to LSF.
  • To identify high-performing clones and strains suitable for enhanced penicillin production in SSF.

Main Methods:

  • Penicillin production was quantified for five P. chrysogenum strains in both SSF and LSF.

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  • Relative production was calculated as the ratio of penicillin production in SSF to LSF (PS/PL).
  • Clones were isolated from each strain and their relative production was subsequently evaluated.

    • Main Results:

    • Strains exhibited varying relative productions (1.4–2.5), while clones showed a wider range (0.6–16.7).
    • Highest LSF producers were also highest SSF producers, indicating production potential is critical for SSF success.
    • High-yielding strains (P2, ASP-78) generated overproducing clones (9,500–10,500 µg/g), but higher LSF producers had lower relative SSF performance.
    • Significant production increases of 500–600% were achieved in SSF with selected overproducing clones.

    Conclusions:

    • Strain production potential is a significant factor influencing penicillin yield in SSF.
    • Higher penicillin-producing strains in LSF may exhibit less efficient expression in SSF.
    • This study successfully identified overproducing clones specifically suited for SSF, leading to substantial production enhancements.