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Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow
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Immunoaffinity purification: basic principles and operational considerations

M L Yarmush1, A M Weiss, K P Antonsen

  • 1Department of Chemical and Biochemical Engineering and the Center for Advanced Biotechnology and Medicine, Rutgers University, Piscataway, NJ 08855, USA.

Biotechnology Advances
|January 1, 1992
PubMed

Immunoaffinity purification has become an important technique in biotechnology. In this review the basic principles of immunoaffinity separations are described with respect to the stages of operation and potential application. The most commonly used support materials, activation procedures, and coupling chemistries are compared to one another for suitability in various applications. Individual operational steps for fixed bed immunoadsorbents including loading, washing, elution and regeneration are described in terms of both theory and practice. Factors influencing adsorbent stability are identified, and alternative operation and configuration strategies are discussed in light of their application to immunoaffinity systems.

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