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Related Experiment Videos

High density cell culture by membrane-based cell recycle.

H N Chang1, I K Yoo, B S Kim

  • 1Department of Chemical Engineering and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology, Taejon, Korea.

Biotechnology Advances
|January 1, 1994
PubMed
Summary

Membrane-based cell recycle enables high cell concentrations in bioreactors, boosting bioprocess productivity. This review compares it to other high-density cell culture systems for metabolite production.

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Area of Science:

  • Biotechnology and Bioprocess Engineering
  • Biochemical Engineering

Background:

  • Conventional bioprocesses (batch, fedbatch, continuous) limit productivity due to low cell concentrations.
  • High biomass concentrations are crucial for enhancing bioprocess productivity.
  • Membrane-based cell recycle is a key technology for achieving high cell densities.

Purpose of the Study:

  • To review and compare membrane-based cell recycle systems with other high-density cell culture methods.
  • To present a theoretical model for metabolite production in membrane-based recycle systems.
  • To discuss the operational aspects of membrane-based cell recycle using fermentation examples.

Main Methods:

  • Comparative analysis of different high-density cell culture systems (membrane recycle, immobilized cells, centrifugation, sedimentation).

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  • Theoretical modeling of primary and secondary metabolite production.
  • Review of operational data from aerobic and anaerobic fermentations utilizing membrane-based cell recycle.

    • Main Results:

    • Membrane-based cell recycle effectively maintains high cell concentrations, surpassing conventional methods.
    • Theoretical framework provided for understanding metabolite yields in these systems.
    • Demonstrated applicability in both aerobic and anaerobic fermentation processes.

    Conclusions:

    • Membrane-based cell recycle is a superior strategy for achieving high cell densities and enhancing bioprocess productivity.
    • The technology offers a viable approach for efficient metabolite production in continuous bioreactor operations.
    • Further research and application in diverse fermentation processes are warranted.