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Related Experiment Videos

Modelling oral malodour in a longitudinal study.

J Pratten1, M Pasu, G Jackson

  • 1Division of Infection and Immunity, Eastman Dental Institute for Oral Health Care Sciences, University College London, 256 Gray's Inn Road, London WC1X 8LD, UK. jpratten@eastman.ucl.ac.uk

Archives of Oral Biology
|October 11, 2003
PubMed
Summary
This summary is machine-generated.

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This study developed an in vitro tongue biofilm model to test anti-malodour compounds. The model effectively assessed volatile sulphur compound reduction and bacterial changes, aiding pre-clinical evaluation.

Area of Science:

  • Oral Microbiology
  • Biotechnology

Background:

  • Oral malodour is a common concern, often linked to volatile sulphur compounds (VSCs) produced by tongue microflora.
  • Assessing anti-malodour agents requires reliable models that mimic the oral environment.

Purpose of the Study:

  • To develop and validate an in vitro model of tongue microflora for evaluating anti-malodour compounds.
  • To assess the efficacy of compounds in reducing VSCs and altering bacterial composition.

Main Methods:

  • A constant depth film fermentor (CDFF) was used to cultivate tongue biofilms in artificial saliva.
  • Bacterial composition was analyzed using differential agars.
  • Volatile sulphur compound (VSC) production was measured with a halimeter.

Main Results:

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  • The model established a representative tongue biofilm with significant Gram-negative anaerobes and H2S-producing bacteria.
  • Pulsing with chlorhexidine or zinc acetate temporarily reduced H2S-producing bacteria and VSCs.
  • Sustained pulsing led to a reduction in VSCs and a shift towards fewer H2S-producing bacteria.

Conclusions:

  • The in vitro CDFF model provides a viable system for assessing anti-malodour compounds.
  • The model demonstrates the potential of certain compounds to reduce VSCs by altering the oral biofilm composition.