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Intermediate filament integrity is required for Junin virus replication
Sandra M Cordo1, Nélida A Candurra
1Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón 2, Piso 4, 1428, Buenos Aires, Argentina.
Virus Research
|October 11, 2003
Summary
Disrupting intermediate filaments (IF) inhibits Junin virus (JUNV) replication without harming cells. IF integrity is crucial for JUNV multiplication after virus entry.
Area of Science:
- Virology
- Cell Biology
- Infectious Diseases
Background:
- The role of microfilaments (MF) and microtubules (MT) in Junin virus (JUNV) replication is known.
- The involvement of intermediate filaments (IF) in JUNV infection remains largely uncharacterized.
Purpose of the Study:
- To investigate the role of intermediate filaments (IF) in Junin virus (JUNV) replication.
- To determine if disrupting IF affects JUNV production and viral protein expression.
Main Methods:
- Treated Vero cells, murine astrocytes, and human foreskin fibroblasts with acrylamide to selectively disrupt IF.
- Assessed JUNV production, cell viability, viral adsorption, internalization, and protein expression.
- Utilized immunofluorescence staining to visualize IF network disruption and protein aggregation.
Main Results:
- Acrylamide treatment inhibited JUNV production in a dose-dependent manner (0.5-3mM) without affecting cell viability.
- Viral adsorption and internalization were not impacted by IF disruption.
- JUNV protein expression was significantly reduced with 2mM acrylamide, and IF networks showed disruption with cytoplasmic aggregates of vimentin or GFAP.
Conclusions:
- Intermediate filaments (IF) play a role in early JUNV multiplication, following virus entry.
- IF network integrity is essential for normal JUNV replication in neural, fibroblast, and Vero cells.