Related Experiment Video
Updated: Jul 10, 2026

Expression of Recombinant Proteins in the Methylotrophic Yeast Pichia pastoris
Published on: February 26, 2010
Overexpression of juvenile hormone binding protein in bacteria and Pichia pastoris
Krystyna Grzelak1, Barbara Kłdkiewicz, Larissa I Kolomiets
1Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.
Abstract:
Galleria mellonella juvenile hormone binding protein (JHBP) is a single chain glycoprotein with two disulfide bonds and a molecular mass of 25,880 Da. This report describes the expression of JHBP in bacteria and yeast cells (Pichia pastoris). The expression in bacteria was low and the protein was rapidly degraded upon cell lysis. The expression of His8-tagged rJHBP (His8-rJHBP) in P. pastoris was high and the non-degraded protein was purified to homogeneity with high yield in a one-step immobilized Ni++ affinity chromatography. His8-rJHBP from P. pastoris contains one JH III binding site with KD of 3.7 +/- 1.3x10(-7) M. The results suggest that P. pastoris is the preferred system for expression of His8-rJHBP in non-degraded fully active form.
Related Concept Videos
Prokaryotic Transcriptional Activators and Repressors
Transcription of prokaryotic...
Transcription Attenuation in Prokaryotes
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
Prokaryotic Transcriptional Activators and Repressors
Transcription of prokaryotic...
Gene Regulation in Microbial Communities: Quorum Sensing
Stringent Response in E. coli
Other Stress Responses in Bacteria

