Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

The Bone Matrix

The Bone Matrix

Bone contains a relatively small number of cells entrenched in a matrix of collagen fibers that provide an adherent surface for inorganic salt crystals. Both components of the matrix, organic and inorganic, contribute to the unusual properties of bone. Without collagen, bones would be brittle and shatter easily. Without mineral crystals, bones would flex and provide little support. This can be observed by an experiment: when the minerals of a bone are dissolved by soaking the bone in acid or...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Ultrastructural changes in cryopreserved tracheal grafts of sprague-dawley rats.

ASAIO journal (American Society for Artificial Internal Organs : 1992)·2009

Same author

Preparation of albumin nanospheres loaded with gemcitabine and their cytotoxicity against BXPC-3 cells in vitro.

Acta pharmacologica Sinica·2009

Same author

Facile synthesis of size-tunable micro-octahedra via metal-organic coordination.

Chemical communications (Cambridge, England)·2009

Same author

N-acetyl cysteine and penicillamine induce apoptosis via the ER stress response-signaling pathway.

Molecular carcinogenesis·2009

Same author

Targeting glucosylceramide synthase downregulates expression of the multidrug resistance gene MDR1 and sensitizes breast carcinoma cells to anticancer drugs.

Breast cancer research and treatment·2009

Same author

N-glycosylation of ATF6beta is essential for its proteolytic cleavage and transcriptional repressor function to ATF6alpha.

Journal of cellular biochemistry·2009

Search research articles

Related Experiment Video

Updated: Jun 29, 2026

Analysis of Minerals Produced by hFOB 1.19 and Saos-2 Cells Using Transmission Electron Microscopy with Energy Dispersive X-ray Microanalysis

Analysis of Minerals Produced by hFOB 1.19 and Saos-2 Cells Using Transmission Electron Microscopy with Energy Dispersive X-ray Microanalysis

Published on: June 24, 2018

[Component analysis of bone matrix via cell extraction].

Hao Wang¹, Shu-ling Bai

¹Department of Tissue Engineering, China Medical University, Shenyang, Liaoning, P. R. China 110001.

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi = Zhongguo Xiufu Chongjian Waike Zazhi = Chinese Journal of Reparative and Reconstructive Surgery

|October 14, 2003

Summary

This summary is machine-generated.

This study details an effective method for preparing bone acellular extra-cellular matrix (AECM), preserving its natural collagen structure and key components like fibronectin and laminin.

More Related Videos

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

Published on: April 12, 2019

Comprehensive Characterization of Tissue Mineralization in an Ex Vivo Model

Comprehensive Characterization of Tissue Mineralization in an Ex Vivo Model

Published on: September 27, 2024

Related Experiment Videos

Last Updated: Jun 29, 2026

Analysis of Minerals Produced by hFOB 1.19 and Saos-2 Cells Using Transmission Electron Microscopy with Energy Dispersive X-ray Microanalysis

Analysis of Minerals Produced by hFOB 1.19 and Saos-2 Cells Using Transmission Electron Microscopy with Energy Dispersive X-ray Microanalysis

Published on: June 24, 2018

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

Published on: April 12, 2019

Comprehensive Characterization of Tissue Mineralization in an Ex Vivo Model

Comprehensive Characterization of Tissue Mineralization in an Ex Vivo Model

Published on: September 27, 2024

Area of Science:

Biomaterials Science
Tissue Engineering
Cell Biology

Context:

Bone tissue engineering relies on scaffolds that mimic native tissue structure and composition.
Acellular extra-cellular matrix (AECM) offers a promising biomaterial for regenerative medicine.
Characterizing AECM is crucial for understanding its potential applications.

Purpose:

To develop and validate a method for preparing bone acellular extra-cellular matrix (AECM).
To analyze the structural and compositional integrity of the prepared bone AECM.
To confirm the presence of essential extracellular matrix components.

Summary:

Bone AECM was prepared using low-osmosis theory and detergent-based cell extraction.
Morphological analysis via HE, Mallory-Heidenhain, and Alcian blue staining revealed regular collagen fiber arrangement and proteoglycan presence.
Immunohistochemistry confirmed the retention of fibronectin (FN) and laminin (LN) within the AECM.

Impact:

The described method effectively produces bone AECM while preserving native collagen structure.
This technique maintains crucial ECM components, including proteoglycans, fibronectin, and laminin.
The findings support the use of this AECM in bone regenerative applications.