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Stored platelets release nucleotides as inhibitors of platelet function
R Fijnheer1, M N Boomgaard, A J van den Eertwegh
1Central Laboratory of The Netherlands Red Cross Blood Transfusion Service, Amsterdam.
Thrombosis and Haemostasis
|November 10, 1992
Summary
Stored platelets release inhibitory nucleotides, impairing platelet function. Resuspending in fresh plasma partially restores function, suggesting a reversible mechanism contributing to storage-related platelet dysfunction.
Area of Science:
- Hematology
- Transfusion Medicine
- Biochemistry
Background:
- Platelet function declines during storage of platelet concentrates at room temperature.
- Understanding the mechanisms behind this decline is crucial for improving transfusion efficacy.
Purpose of the Study:
- To investigate the detailed mechanisms of platelet function decrease during storage.
- To identify factors in stored platelet concentrates that inhibit platelet function.
Main Methods:
- Resuspending stored platelets in fresh plasma to assess recovery of aggregation and ATP secretion.
- Incubating fresh platelets in plasma from stored concentrates to evaluate inhibitory effects.
- Storing platelets in plasma-free media and analyzing the supernatant for inhibitory substances.
Main Results:
- Stored platelets showed partial recovery of function after resuspension in fresh plasma.
- Fresh platelets incubated in stored plasma exhibited significantly reduced function.
- Supernatants from platelets stored in plasma-free media inhibited fresh platelet function in a time-dependent manner.
- AMP and diadenosine tetraphosphate were identified as inhibitory substances in the supernatant.
Conclusions:
- Stored platelets release inhibitory nucleotides, such as AMP and diadenosine tetraphosphate.
- These released nucleotides reversibly inhibit platelet function, contributing to storage-related dysfunction.
- This finding may explain partial recovery of platelet function after transfusion.