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Karyotyping of human metaphase II oocytes by multifluor fluorescence in situ hybridization
Julie M Clyde1, Jan E Hogg, Anthony J Rutherford
1Reproductive Biology Group, Academic Unit of Paediatrics, Obstetrics, and Gynaecology, University of Leeds, Leeds, United Kingdom.
Objective:
To quantify aneuploidy in inseminated, injected, and noninjected oocytes from infertility patients using Multifluor fluorescence in situ hybridization (M-FISH).
Design:
Prospective study.
Setting:
Reproductive biology group, academic unit of pediatrics, obstetrics, and gynecology.
Patient(S):
Forty-eight patients undergoing ovarian stimulation and either intracytoplasmic sperm injection (ICSI) or conventional in vitro fertilization (IVF).
Intervention(S):
None.
Main Outcome Measure(S):
M-FISH karyotyping of 67 metaphase II oocytes, including noninjected in vitro matured oocytes, and injected inseminated-failed fertilized oocytes.
Result(S):
Thirty-nine percent of oocytes were aneuploid, with nondisjunction of chromosomes in 34% of oocytes and predivision of chromatids in 10%. There was no difference in aneuploidy rates between ICSI noninjected in vitro matured oocytes and injected, failed fertilized oocytes. Chromosomes most frequently involved in aneuploidy were 15, 18, 19, 22, and X. In seven injected ICSI MII oocytes, the prematurely condensed sperm chromatin was karyotyped by M-FISH.
Conclusion(S):
M-FISH was used to diagnose aneuploidy at maternal meiosis I in 39% of oocytes, and M-FISH karyotyping of sperm was demonstrated.
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