Related Experiment Videos
Human chymase stimulates Ca2+ signaling in human polymorphonuclear cells
Kayo Saito1, Tsuyoshi Muto, Yoshiaki Tomimori
1Suntory Biomedical Research Limited, 1-1-1 Wakayamadai, Shimamoto-cho, Mishima-gun, 618-8503, Osaka, Japan.
Immunology Letters
|October 15, 2003
Summary
Human chymase triggers leukocyte migration by increasing intracellular calcium levels in polymorphonuclear (PMN) cells. This calcium signaling involves both extracellular influx and internal release, mediated by G protein-coupled receptors.
Area of Science:
- Immunology
- Cell Biology
- Biochemistry
Background:
- Human chymase acts as a chemoattractant for leukocytes.
- Understanding the mechanism of chymase-induced cell migration is crucial.
Purpose of the Study:
- To investigate the intracellular calcium signaling pathway involved in human chymase-mediated polymorphonuclear (PMN) cell migration.
- To elucidate the role of G protein signaling and calcium dynamics in this process.
Main Methods:
- Measurement of intracellular calcium concentration ([Ca(2+)]i) in human PMN cells using Fluo-3.
- Manipulation of extracellular calcium levels and use of pertussis toxin to assess signaling pathways.
Main Results:
- Human chymase induced a concentration-dependent increase in [Ca(2+)]i in PMN cells.
- The calcium response involved both extracellular Ca(2+) influx and release from internal stores.
- Pertussis toxin treatment abolished the chymase-induced [Ca(2+)]i signal, indicating G protein involvement.
Conclusions:
- Chymase-induced PMN cell migration is likely mediated by intracellular calcium elevation.
- This calcium signaling is dependent on G protein activation, possibly through protease-activated receptors (PARs).