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Related Experiment Videos

Hydrodynamic interactions between rolling leukocytes in vivo.

Michael R King1, Michael B Kim, Ingrid H Sarelius

  • 1Department of Biomedical Engineering, University of Rochester, Rochester, NY 14642, USA. mike_king@urmc.rochester.edu

Microcirculation (New York, N.Y. : 1994)
|October 15, 2003
PubMed
Summary

Leukocyte rolling velocity and adhesion dynamics in venules are significantly influenced by nearby cells. Increased local leukocyte concentration promotes further cell adhesion, highlighting multicellular effects in microcirculation.

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Area of Science:

  • Immunology
  • Biophysics
  • Microcirculation

Background:

  • Leukocyte adhesion is crucial for immune responses.
  • Selectin-mediated rolling is a key initial step in leukocyte extravasation.
  • Hydrodynamic interactions in microvessels are complex and not fully understood.

Purpose of the Study:

  • To characterize hydrodynamic interactions between rolling and free-stream leukocytes.
  • To identify physical mechanisms governing leukocyte adhesion dynamics in postcapillary venules.
  • To investigate the role of local leukocyte concentration in adhesion.

Main Methods:

  • Intravital microscopy of postcapillary venules (22-37 microm) in hamster cheek pouches.
  • Induction of selectin-mediated leukocyte rolling via surgical preparation.

Related Experiment Videos

  • Analysis of rolling velocity and cell-cell interactions.

    • Main Results:

    • Rolling velocity strongly correlated with the separation to the nearest cell and the number of nearby cells.
    • Adherent leukocytes acted as nucleation sites, promoting further free-stream cell adhesion.
    • Observed effects exceeded those due to vessel length variations.

    Conclusions:

    • Leukocyte adhesion dynamics in venules are heavily influenced by local adherent leukocyte concentration.
    • Complex hydrodynamics induced by multiple cells play a significant role.
    • Further research into multicellular effects in the microcirculation is warranted.