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Zygotic Fluorescence Recovery After Photo-bleaching Analysis for Chromatin Looseness That Allows Full-term Development
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Ab initio excited-state dynamics of the photoactive yellow protein chromophore

Chaehyuk Ko1, Benjamin Levine, A Toniolo

  • 1Department of Chemistry and The Beckman Institute, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.

Journal of the American Chemical Society
|October 16, 2003
PubMed

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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...