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Microplate based biosensing with a computer screen aided technique.

Daniel Filippini1, Tony P M Andersson, Samuel P S Svensson

  • 1Division of Applied Physics, Institute of Physics and Measurement Technology, Linköping University, SE-581 83 Linköping, Sweden. danfi@ifm.liu.se

Biosensors & Bioelectronics
|October 16, 2003
PubMed
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This study introduces a novel, affordable microplate reader using a computer screen and webcam. The system successfully detects the asthma medication formoterol in blood, offering a versatile platform for various bioassays.

Area of Science:

  • Cell Biology
  • Biophysics
  • Analytical Chemistry

Background:

  • Melanophores from Xenopus laevis exhibit light absorbance changes upon stimulation.
  • Hormonal agents like melatonin and alpha-melanocyte stimulating hormone serve as reversible stimuli.
  • Existing commercial systems for bioassays can be expensive and highly dedicated.

Purpose of the Study:

  • To test a new compact microplate reading platform.
  • To demonstrate the detection of the asthma drug formoterol in blood plasma.
  • To evaluate the versatility and broad applicability of the developed platform for bioassays.

Main Methods:

  • Utilized frog melanophores as a biological model.
  • Employed hormone exposure (melatonin, alpha-melanocyte-stimulating hormone) as a stimulus.

Related Experiment Videos

  • Developed a system using a computer screen as a light source and a webcam as a detector for microplate reading.
  • Enabled kinetic microplate reading capabilities.

    • Main Results:

    • Successfully demonstrated the detection of formoterol in blood plasma samples.
    • Validated the platform's ability to perform reversible measurements upon hormone stimulation.
    • Showcased the system's potential for kinetic measurements.

    Conclusions:

    • The developed platform is a versatile, broadly available, and cost-effective alternative to commercial systems.
    • This system is particularly advantageous for point-of-care testing and self-testing applications.
    • The compact microplate reader shows significant potential for evaluating numerous bioassays.