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Optical-transparent and flexible glucose sensor with ITO electrode.
Kohji Mitsubayashi1, Yoshihiko Wakabayashi, Satoshi Tanimoto
1Department of Human and Information Science, School of Engineering, Tokai University, 1117 Kitakaname, Hiratsuka, Kanagawa 259-1292, Japan. mitsu@keyaki.cc.u-tokai.ac.jp
Biosensors & Bioelectronics
|October 16, 2003
Summary
A novel glucose sensor was developed using glucose oxidase (GOD) immobilized on a transparent oxygen electrode. This flexible, optically transparent sensor accurately measures tear glucose levels, showing high reproducibility for potential diagnostic applications.
Area of Science:
- Biomedical Engineering
- Biosensors
- Electrochemistry
Background:
- Accurate glucose monitoring is crucial for diabetes management.
- Existing glucose sensors face limitations in flexibility and optical properties.
Purpose of the Study:
- To develop a novel, flexible, and optically transparent glucose sensor.
- To evaluate the sensor's performance in measuring glucose concentrations, particularly in tear fluid.
Main Methods:
- Immobilization of glucose oxidase (GOD) onto a transparent oxygen electrode using glutaraldehyde.
- Fabrication of the oxygen electrode using Indium-Tin Oxide (ITO) and a gas-permeable membrane.
- Sensor characterization using a computer-controlled potentiostat at -900 mV reduction potential.
Main Results:
- The sensor exhibited flexibility and high optical transparency (less than 0.6 abs from 400-700 nm).
- Accurate glucose measurement range from 0.06 to 1.24 mmol/l with a high correlation coefficient (0.999).
- The sensor successfully measured physiological tear glucose concentrations (0.14 mmol/l) with good reproducibility.
Conclusions:
- The developed glucose sensor is a promising device for non-invasive glucose monitoring.
- Its flexible and transparent nature, coupled with accurate measurement capabilities, supports potential clinical applications.