Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
Regulated Protein Degradation02:58

Regulated Protein Degradation

It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
Septins01:19

Septins

Septins are protein filaments forming the cytoskeleton along with the microtubules, microfilaments, intermediate filaments, and other accessory proteins. In 1971 while studying the cell division cycle in mutant Saccharomyces cerevisiae Harwell et al. first identified the septin-related genes playing a crucial role in yeast cytokinesis. Fluorescence microscopy revealed that these proteins localize at the budding neck as rings. These ring-like proteins were then named Septins by John Pringle, and...
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
Role of Septins01:02

Role of Septins

Septins are the recently discovered fourth major protein component of the cytoskeleton, along with microfilaments, microtubules, and intermediate filaments. These proteins can associate with other cytoskeletal filaments and carry out varied roles or can be free-floating in the cytoplasm.
Cellular Functions of Septins
Recent studies have revealed the multifaceted roles of septins in various cellular processes such as cytokinesis, ciliogenesis, and neurogenesis. Septins act as scaffolds and...
Regulated Protein Degradation02:58

Regulated Protein Degradation

It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Label-free electrochemical levodopa detection via dummy imprinted polymers for advanced disease monitoring.

Analytica chimica acta·2026
Same author

A Case Report of a Novel Atypical Mycobacterial Infection: Mycobacterium Chimaera Hand Tenosynovitis.

JBJS case connector·2022
Same author

The Institute on Methods and Protocols for Advancement of Clinical Trials in ADRD (IMPACT-AD): A Novel Clinical Trials Training Program.

The journal of prevention of Alzheimer's disease·2021
Same author

Papillary glioneuronal tumors: Distinctive cytological characteristics and cyto-histologic correlation.

Annals of diagnostic pathology·2021
Same author

Progressing Bevacizumab-Induced Diffusion Restriction Is Associated with Coagulative Necrosis Surrounded by Viable Tumor and Decreased Overall Survival in Patients with Recurrent Glioblastoma.

AJNR. American journal of neuroradiology·2016
Same author

Silicon detectors for combined MR-PET and MR-SPECT imaging.

Nuclear instruments & methods in physics research. Section A, Accelerators, spectrometers, detectors and associated equipment·2013

Related Experiment Video

Updated: Jul 13, 2026

Optimized Protocol for the Extraction of Proteins from the Human Mitral Valve
09:13

Optimized Protocol for the Extraction of Proteins from the Human Mitral Valve

Published on: June 14, 2017

SEPT5_v2 is a parkin-binding protein.

P Choi1, H Snyder, L Petrucelli

  • 1Department of Pharmacology, Loyola University Medical Center, Bldg 102/3634, 2160 S 1st Ave, Maywood, IL 60153, USA.

Brain Research. Molecular Brain Research
|October 16, 2003
PubMed
Summary

Parkin, an enzyme linked to Parkinson's disease, binds to and ubiquitinates SEPT5_v2, a protein involved in cell division. This interaction is crucial for the normal metabolism of SEPT5 proteins in the brain.

More Related Videos

Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation
10:21

Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation

Published on: February 1, 2019

Purification and Quality Control of Recombinant Septin Complexes for Cell-Free Reconstitution
11:50

Purification and Quality Control of Recombinant Septin Complexes for Cell-Free Reconstitution

Published on: June 23, 2022

Related Experiment Videos

Last Updated: Jul 13, 2026

Optimized Protocol for the Extraction of Proteins from the Human Mitral Valve
09:13

Optimized Protocol for the Extraction of Proteins from the Human Mitral Valve

Published on: June 14, 2017

Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation
10:21

Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation

Published on: February 1, 2019

Purification and Quality Control of Recombinant Septin Complexes for Cell-Free Reconstitution
11:50

Purification and Quality Control of Recombinant Septin Complexes for Cell-Free Reconstitution

Published on: June 23, 2022

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genetics

Background:

  • Mutations in the parkin gene are linked to inherited forms of Parkinson's disease (PD).
  • Parkin functions as a ubiquitin ligase, targeting proteins for degradation, but its substrates are not fully identified.
  • Septins are a conserved family of GTP-binding proteins involved in various cellular processes.

Purpose of the Study:

  • To identify novel substrates of parkin.
  • To investigate the relationship between parkin and the septin family of proteins.

Main Methods:

  • Yeast two-hybrid screening to identify parkin-interacting proteins.
  • Co-precipitation assays using human substantia nigra lysates.
  • In vitro ubiquitination assays.
  • Analysis of protein levels in brains of patients with Autosomal Recessive Juvenile Parkinsonism (ARJP).

Main Results:

  • Human SEPT5_v2 was identified as a putative parkin-binding protein.
  • SEPT5_v2 binds to parkin's amino terminus and ring finger domains.
  • Parkin co-precipitates with SEPT5_v2 and ubiquitinates it in vitro.
  • SEPT5_v1 and SEPT5_v2 accumulate in the brains of ARJP patients, indicating parkin's role in their metabolism.

Conclusions:

  • A significant relationship exists between parkin and septins, specifically SEPT5_v2.
  • Parkin-mediated ubiquitination is essential for the normal metabolism of SEPT5 proteins.
  • These findings provide new insights into the molecular mechanisms underlying Parkinson's disease.