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Type 1 angiotensin II receptor-associated protein ARAP1 binds and recycles the receptor to the plasma membrane

Deng-Fu Guo1, Isabelle Chenier, Valerie Tardif

  • 1Research Centre, Hôtel-Dieu du CHUM, Department of Medicine, Université de Montréal, Montréal, Québec, Canada H2W 1T8. guod@magellan.umontreal.ca

Biochemical and Biophysical Research Communications
|October 16, 2003
PubMed

Insights

A novel protein, ARAP1, facilitates the recycling of the type 1 angiotensin II receptor (AT(1)) back to the cell surface. This recycling restores the receptor

Area of Science:

  • Molecular Cell Biology
  • Receptor Trafficking and Signaling

Background:

  • The carboxyl terminus of the type 1 angiotensin II receptor (AT(1)) is crucial for its regulation, including phosphorylation, desensitization, and internalization.
  • Understanding proteins that interact with AT(1) is key to deciphering its cellular fate and function.

Purpose of the Study:

  • To identify proteins interacting with the carboxyl terminus of the AT(1A) receptor using a yeast two-hybrid system.
  • To characterize the function of a newly identified protein, ARAP1, in AT(1A) receptor trafficking.

Main Methods:

  • Yeast two-hybrid screening to identify interacting proteins.
  • Immunoprecipitation and Western blotting to confirm protein complex formation.
  • HEK-293 cell culture for functional assays and immunocytochemistry.
  • Calcium (Ca2+) release assays to measure receptor activity.
  • Immunocytochemical analysis for co-localization studies.

Main Results:

  • Isolation and identification of a novel protein, Angiotensin II Receptor Associated Protein 1 (ARAP1).
  • ARAP1 forms a complex with the AT(1A) receptor in HEK-293 cells.
  • ARAP1 promotes the recycling of AT(1A) to the plasma membrane, restoring its responsiveness to Angiotensin II.
  • Co-localization of ARAP1 and recycled AT(1A) at the plasma membrane was observed.

Conclusions:

  • ARAP1 plays a significant role in the recycling of the AT(1) receptor to the plasma membrane.
  • ARAP1-mediated recycling appears to restore the signaling capacity of the AT(1) receptor.
  • This finding sheds light on the regulatory mechanisms governing AT(1) receptor function and trafficking.

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