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Inhibitory effects of grape seed extract on lipases
Diego A Moreno1, Nebojsa Ilic, Alexander Poulev
1Biotech Center, Cook College, Rutgers--The State University of New Jersey, New Brunswick, New Jersey 08901-8520, USA. moreno@aesop.rutgers.edu
Objective:
The aim of the present study was to assess the effects of grape seed extract (GSE) on the fat-metabolizing enzymes pancreatic lipase, lipoprotein lipase, and hormone-sensitive lipase in vitro and evaluate its potential application as a treatment for obesity.
Methods:
Crushed grape seeds were extracted in ethanol, and the extract was assayed for the measurement of inhibitory effects on pancreatic lipase and lipoprotein lipase activities and on lipolysis of 3T3-L1 adipocytes.
Results:
The GSE rich in bioactive phytochemicals showed inhibitory activity on the fat-metabolizing enzymes pancreatic lipase and lipoprotein lipase, thus suggesting that GSE might be useful as a treatment to limit dietary fat absorption and the accumulation of fat in adipose tissue. The observed reduction in intracellular lipolytic activity of cultured 3T3-L1 adipocytes may reduce the levels of circulating free fatty acids that have been linked to insulin resistance in obese patients.
Conclusion:
The GSE rich in compounds that inhibit lipases may provide a safe, natural, and cost-effective weight control treatment.
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