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Updated: Aug 9, 2026
Flow Cytometry Analysis of Tissue Factor Expression in Human Platelets
Published on: November 22, 2024
[Detection of platelet activation by flow cytometry in patients with pre-eclampsia]
Zheng-Shan Yi1, Shu-Yun Zhou, Fan-Yi Meng
1Department of Hematology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
Objective:
To explore the clinical significance of expression alterations of static and activated platelet glycoprotein CD41a, CD62p, and CD63 in patients with pre-eclampsia.
Methods:
We used non-washing immunolabeling technique for fluorescence labeling of the static and activated platelet with CD41a, CD62p, and CD63 monoclonal antibodies of the blood samples from 16 patients with pre-eclampsia, 15 women with normal pregnancies and 20 healthy subjects. The expression of the monoclonal antibody was detected by flow cytometry.
Results:
The average static and activated platelet glycoprotein CD41a, CD62p, and CD63 expression levels in patients with pre-eclampsia were apparently higher than those of the normal pregnant women (P<0.01), while the comparison between the latter and the control group did not reveal any significant differences (P>0.05).
Conclusion:
High levels of platelet glycoprotein CD62p and CD63 expressions are present in patients with preeclampsia, suggesting the presence of platelet activation in these patients.
